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Estimation of the p and m Isomers of Hydroxyphenylacetic Acid in Mouse Brain by a Gas Chromatographic Procedure: Their Regional Distribution and the Effects of Some Drugs
Author(s) -
McQuade P. S.,
Juorio A. V.,
Boulton A. A.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb12549.x
Subject(s) - chemistry , chromatography , sephadex , monoamine oxidase , tyramine , gas chromatography , derivatization , high performance liquid chromatography , biochemistry , enzyme
The mouse brain contains 12.5 and 4.1 ng/g of p‐ and m ‐hydroxyphenylacetic acids, respectively. The hydroxyphenylacetic acids were isolated by chromatography on DEAE‐Sephadex A‐25 and quantitated as their pentafluoropropionyl and hexafluoropropanol esters by use of a gas chromatograph equipped with an electron‐capture detector. The highest concentrations of p‐ or m ‐hydroxyphenylacetic acids were observed in the caudate nuclei (27.9 and 8.7 ng/g, respectively) and olfactory tubercles (20.2 and 5.3 ng/g, respectively). The identities of the p‐ and m ‐hydroxyphenylacetic acids were further confirmed as a consequence of the reductions observed following monoamine oxidase inhibition or the increases observed in the appropriate acid following the parenteral administration of p‐ or m ‐tyramine.