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Striatal Dopamine Release In Vitro : A β‐Adrenoceptor‐Regulated Response Not Mediated Through Cyclic AMP
Author(s) -
Reisine Terry,
Chesselet MarieFrancoise,
Glowinski Jacques
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb11485.x
Subject(s) - ibmx , efflux , endocrinology , medicine , dopamine , chemistry , agonist , phosphodiesterase inhibitor , phosphodiesterase , tyrosine hydroxylase , biology , receptor , forskolin , biochemistry , enzyme , stimulation
The effects of (‐)isoproterenol(10 6 M), dibutyryl cyclic AMP (10 −2 M), and the phosphodiesterase inhibitor 3‐isobutyl‐1‐methylxanthine (IBMX) (10 −4 M) on in vitvo [ 3 H]dopamine ([ 3 H]DA) cllux and synthesis were studied in rat striatal slices continuously superfused with [ 3 H]tyrosine. The β‐adrenoceptor agonist (‐)isoproterenol induced an immediate and significant facilitation of [ 3 H]DA efflux but did not alter [ 3 H]DA synthesis as measured by [ 3 H]H 2 O formation. In contrast, both dibutyryl cyclic AMP and IBMX enhanced [ 3 H]DA synthesis as well as efflux. The presence of IBMX in the superfusing medium did not potentiate the augmentation of [ 3 H]DA efflux caused by (‐)isoproterenol. Additionally, the blockade of [ 3 H]DA synthesis by α‐methyl‐ p ‐tyrosine (10 −1 M) completely prevented the action of dibutyryl cyclic AMP on [ 3 H]DA efflux. However, under similar conditions, (‐)so proterenol was still able to increase [ 3 H]DA efflux. The results suggest that (‐)isoproterenol can modify striatal DA release through a mechanism not involving cyclic AMP.