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“On” and “Off” Responses of K + ‐Induced Synaptosomal Proline Release: Involvement of the Sodium Pump
Author(s) -
Nickolson Victor J.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb10885.x
Subject(s) - sodium , chemistry , proline , sodium pump , biophysics , biochemistry , biology , amino acid , organic chemistry , ouabain
The K + ‐induced release of the putative neurotransmitter proline in rat cerebral cortex synaptosomes was studied by measuring [5‐ 3 H]proline released upon exposure to high‐K + media. In contrast to previous experiments, K + was not substituted for Na + , which was held constant. Under the resulting conditions of increased osmolarity, two peaks of proline release were obtained, one upon exposure to increased [K + ], another upon restoring normal [K + ]. The dependence of the latter peak on ionic composition of the medium, and its sensitivity to ouabain are discussed.