Premium
[ 14 C]Glucose Metabolism in Sympathetic Ganglia of Chicken Embryos and in Primary Cultures of Neurons and of Other Cells from These Ganglia
Author(s) -
Larrabee Martin G.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb10874.x
Subject(s) - embryo , biology , incubation , metabolism , tissue culture , carbohydrate metabolism , in vitro , biochemistry , endocrinology , medicine , microbiology and biotechnology
Metabolism of [1‐ 14 C]glucose and [6‐ 14 C]glucose was measured in sympathetic ganglia excised from chicken embryos 12–16 days old and in primary cultures of neurons or nonneurons prepared from these ganglia. Some metabolic rates tended to change with the tissue/medium ratio, so this variable had to be controlled. Less C‐6 than C‐1 of glucose was put out in CO 2 by all three types of preparations, indicating operation of the hexosemonophosphate shunt. The C‐6/C‐1 ratio was greater for the neuronal cultures and for intact ganglia than for the nonneuronal cultures. The C‐6/C‐1 ratio for the neurons increased with the amount of tissue added to a given volume of incubation medium, in agreement with previous experiments on embryonic dorsal root ganglia (Larrabee, 1978). Per unit of protein, the output of C‐1 of glucose in CO 2 was higher in both the neuronal and the nonneuronal cultures than in intact ganglia, whereas that of C‐6 was higher in the neuronal cultures and lower in the nonneuronal ones than in the ganglia. The rates of release in lactate of C‐1 and C‐6 of glucose were 3–5 times higher from both types of cultures than from intact ganglia. The average rates of incorporation of C‐1 and C‐6 of glucose into tissue constituents were lower in the cultures than in intact ganglia, significantly so for incorporation of C‐6 in the nonneuronal cultures.