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Postmortem Accumulation of Tubulin in Postsynaptic Density Preparations
Author(s) -
Carlin Richard K.,
Grab Dennis J.,
Siekevitz Philip
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb10858.x
Subject(s) - tubulin , postsynaptic density , postsynaptic potential , forebrain , cerebral cortex , coomassie brilliant blue , biology , microtubule , cerebellum , excitatory postsynaptic potential , biophysics , biochemistry , chemistry , microbiology and biotechnology , central nervous system , neuroscience , staining , receptor , genetics
Postsynaptic density (PSD) preparations isolated from canine cerebral cortex that had been left at 0–37°C for various times were found to become enriched in two bands in a time‐ but not temperature‐dependent manner. The two bands were identified as tubulin subunits by gel mobility and immunology. Of all the isolated synaptic structures the increase in tubulin occurred primarily in the PSD fraction. The increase of tubulin also occurred in PSD preparations isolated from canine cerebellum and rat forebrain. Results obtained when PSD fractions were isolated from canine brain obtained as rapidly as possible after the death of the animal indicate that the maximum amount of tubulin in the PSD preparations is 2.5% of total Coomassie blue‐stained protein as determined by scanning of gel electrophoretograms. These results imply that tubulin is probably not a major structural protein of the PSD as it exists in situ.