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Effects of Cap Analogue or Cap Removal on the Translation of Rat Brain mRNA In Vitro
Author(s) -
Murthy M. R.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb10851.x
Subject(s) - translation (biology) , in vitro , messenger rna , chemistry , neuroscience , microbiology and biotechnology , biology , biochemistry , gene
The role of cap structures in the translation of brain mRNA was examined by measuring protein biosynthesis in vitro in wheat germ and reticulocyte systems programmed by mRNA that was either untreated or oxidized by periodate or from which 5′‐terminal 7‐methylguanosine (m 7 G) was removed by oxidation and β ‐elimination. In another series of reactions, amino acid incorporation into polypeptides was measured in the absence and in the presence of varying concentrations of the cap analogue 7‐methylguanosine 5′‐triphosphate (pppm 7 G). The results indicated that any of the above treatments interfered with brain mRNA translation, the degree of inhibition depending on the translation system used, the concentration of mRNA, and the source of initiation factors. Homologous brain initiation factors were superior to reticulocyte factors in providing a partial relief from inhibition of translation caused by these treatments. It was also found that synthesis of the brain‐specific protein S‐100 was inhibited by β ‐elimination of mRNA, by pppm 7 G, or by the presence of capped globin mRNA, indicating that the mRNA for this protein was probably capped.