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Activation of Brain Guanylate Cyclase by Phospholipase A 2
Author(s) -
Zwiller J.,
CiesielskiTreska J.,
Ulrich G.,
Revel M. O.,
Mandel P.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08712.x
Subject(s) - gucy1a3 , guanylate cyclase , gucy1b3 , guanylate cyclase 2c , gucy2d , chemistry , phospholipase , neuroscience , medicine , biochemistry , biology , enzyme
Various pure snake venom phospholipases A 2 were used for studying their effect on guanylate cyclase activity. All the phospholipases A 2 tested were found to activate guanylate cyclase from a rat brain homogenate. It was shown that particulate guanylate cyclase was especially affected. Intact glial cells incubated in presence of phospholipase A 2 showed also an increased guanylate cyclase activity, demonstrating that the phospholipase effect, observed in disrupted cells, occurs also at the cellular level. These results suggest that in intact cells membrane‐bound phospholipase A 2 activity could be involved in the modulation of the cellular cyclic GMP content.