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Glycoproteins of Axonal Transport: Affinity Chromatography on Fucose‐Specific Lectins
Author(s) -
Gustavsson S.,
Ohlson C.,
Karlsson J. O.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08711.x
Subject(s) - fucose , lectin , glycoprotein , ulex europaeus , chemistry , biochemistry , affinity chromatography , chromatography , agglutinin , enzyme
Rapidly transported fucose‐labeled glycoproteins from axons of rabbit retinal ganglion cells were solubilized with nonionic detergents. The solubilized components were subjected to affinity chromatography on three different fucose‐specific lectins. A recently characterized fucose‐specific lectin from Alcuria aurantia bound reversibly approximately 60% of the applied protein‐bound radioactivity. The lectins from Lotus tetragonolohus and Ulex europaeus bound very small proportions of the labeled rapidly transported glycoproteins.