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Simultaneous Determination of Serotonin, 5‐Hydroxyindoleacetic Acid, 3,4‐Dihydroxyphenylacetic Acid and Homovanillic Acid by High Performance Liquid Chromatography with Electrochemical Detection
Author(s) -
Sperk Günther
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08708.x
Subject(s) - homovanillic acid , 5 hydroxyindoleacetic acid , chromatography , 3,4 dihydroxyphenylacetic acid , serotonin , perchloric acid , chemistry , high performance liquid chromatography , biochemistry , organic chemistry , receptor
A rapid and highly sensitive procedure for simultaneous determination of serotonin, 5‐hydroxyindoleacetic acid, 3,4‐dihydroxyphenylacetic acid and homovanillic acid is described. After precipitation of proteins with perchloric acid the samples are applied directly to a high performance liquid chromatograph, with electrochemical detection. As little as 20 pg of serotonin, 5‐hydroxyindoleacetic acid, and 3,4‐dihydroxyphenylacetic acid and 200 pg of homovanillic acid can be detected. One chromatographic run requires less than 10 min.