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Cellular Level of Purine Compounds in Ischemic Gerbil Brain by High Performance Liquid Chromatography
Author(s) -
Morimoto Kazuyoshi,
Tagawa Kunio,
Hayakawa Torn,
Watanabe Fusao,
Mogami Heitaro
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08706.x
Subject(s) - gerbil , inosine , hypoxanthine , high performance liquid chromatography , ligation , adenosine , purine , ischemia , chromatography , chemistry , carotid arteries , medicine , biochemistry , enzyme
The cellular level of ATP and related compounds in ischemic gerbil brain was investigated by high performance liquid chromatography (HPLC). Brain samples were obtained in situ following ligation of the common carotid artery. AMP and ADP peaks in the brain extracts in the ischemic group became much larger whereas the ATP peak decreased dramatically. The most striking finding was an extensive increase of adenosine: 50–100 fold. The levels of inosine and hypoxanthine also increased greatly in typical symptomatic gerbil.