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Phosphatidylserine Enhancement of [ 3 H]γ‐Aminobutyric Acid Uptake by Rat Whole Brain Synaptosomes
Author(s) -
Chweh A. Y.,
Leslie S. W.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08687.x
Subject(s) - nipecotic acid , phosphatidylserine , phosphatidylethanolamine , chemistry , synaptosome , phosphatidic acid , phosphatidylinositol , biochemistry , gaba transporter , sodium , biophysics , phosphatidylcholine , gabaergic , biology , neurotransmitter , in vitro , phospholipid , membrane , receptor , organic chemistry , kinase
[ 3 H] γ ‐Aminobutyric acid ([ 3 H]GABA) binding to purified lipids was examined in an organic solvent‐aqueous partition system. In addition, the [ 3 H]GABA binding capacity in the partition system was compared with the capacity of lipids to alter sodium‐dependent [ 3 H]GABA uptake into synaptosomes isolated from rat whole brains. [ 3 H]GABA was found to bind to all of the lipids studied in the organic solvent‐aqueous partition system [phosphatidic acid (PA), phosphatidylethanolamine (PE), phosphatidylinositol (PI), phosphatidylserine (PS), gangliosides, and sulfatide], although PS exhibited the greatest binding capacity. [ 3 H]GABA uptake into synaptosomes was enhanced by PS (48.0%) but was not altered by any other lipid. PS enhancement of [ 3 H]GABA uptake required the presence of sodium and was blocked by nipecotic acid (10 μ m ). These results suggest that PS may play a role in the sodium‐dependent GABA reuptake process in the presynaptic nerve end.