Premium
Uptake of Adenosine by Isolated Rat Brain Capillaries
Author(s) -
Wu P. H.,
Phillis J. W.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08686.x
Subject(s) - adenosine , neuroscience , chemistry , biology , biochemistry
Adenosine uptake by isolated rat brain capillaries is a carrier‐mediated, temperature‐ and pH‐sensitive process. The K m value for adenosine uptake is 4.74 μ m and the V max is 21.7 picomol/mg protein/10 min. This is a high‐affinity uptake system that can be cross‐inhibited by several nucleosides and by the adenosine analogs tubercidin and 5′‐deoxyadenosine. The uptake is very sensitive to inhibition by papaverine, hexobendine, and dipyridamole. These results confirm the existence of a nucleoside transport system associated with the blood‐brain barrier observed during in vivo studies.