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Active Transport of Nicotine by the Isolated Choroid Plexus In Vitro
Author(s) -
Spector Reynold,
Goldberg Mark J.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08669.x
Subject(s) - choroid plexus , nicotine , in vivo , choroid , chemistry , plexus , in vitro , pharmacology , endocrinology , anatomy , biochemistry , medicine , biology , central nervous system , neuroscience , retina , microbiology and biotechnology
In vitro , the transport of [ 14 C]nicotine into the isolated choroid plexus, the anatomical locus of the blood–CSF barrier, was studied. The isolated rabbit choroid plexus accumulated [ 14 C]nicotine by two processes: an active saturable transport process and a nonsaturable process. The [ 14 C]nicotine accumulation process by choroid plexus was not due to binding or intracellular metabolism of the [ 14 C]nicotine. The [ 14 C]nicotine accumulation process in isolated choroid plexus was inhibited by weak bases, including tolazoline and lidocaine, but not by the weak acid probenecid. The accumulation process was decreased 60% by iodoacetate and dinitrophenol and by low temperatures. These results are consistent with previous autoradiographic evidence showing the choroid plexus concentrated [ 14 C]nicotine in vivo , and suggest that the choroid plexus may transfer nicotine between blood and CSF in vivo .