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Adenosine‐Elicited Accumulation of Adenosine 3′, 5′‐Cyclic Monophosphate in the Chick Embryo Retina
Author(s) -
Carvalho R. Paes,
Mello F. G.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08655.x
Subject(s) - ibmx , adenosine , retina , endocrinology , medicine , phosphodiesterase inhibitor , adenosine deaminase , biology , phosphodiesterase , guanosine , stimulation , amp deaminase , chemistry , biochemistry , enzyme , forskolin , neuroscience
The cyclic AMP level of 17‐day‐old chick embryo retina increased from 20 to 331 pmol/mg protein when the tissue was incubated for 20 min in the presence of 4‐(3‐butoxy‐4‐methoxybenzyl‐2‐imidozolinone) (RO 20‐1724). The addition of 0.5 m m ‐3‐isobutyl‐1‐methylxanthine (IBMX) or 0.5 units/ml of adenosine deaminase (EC 3.5.4.4) to the medium reduced the increase of cyclic AMP content from 20 to 100 pmol/mg protein. Dipyridamole did not interfere with the rise of the retina cyclic AMP level observed with RO 20‐1724. The EC 50 of 6‐amino‐2‐chloropurine riboside (2‐chloroadenosine)‐elicited accumulation of cylic AMP of retinas incubated in the presence of RO 20‐1724 plus adenosine deaminase was approximately 1 μ m . When retina incubation was carried out in the presence of 0.5 m m ‐IBMX, the 2‐chloroadenosine dose–response curve was shifted to the right two orders of magnitude. Maximal stimulation of the cyclic AMP level of 17‐day‐old chick embryo retina incubated in the presence of 0.5 m m ‐IBMX was observed at 1 m m ‐adenosine concentration. This effect was not blocked by dopamine antagonists. Guanosine and adenine did not affect the retinal cyclic AMP level. AMP and ATP had a slight stimulatory effect. Adenosine response of embryonic retina increased sharply from the 14th to the 17th embryonic day. A similar, but not identical, adenosine effect was observed in cultured retina cells.