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Production of [ 14 C]Acetylcholine and [ 14 C]Carbon Dioxide from [U– 14 C]Glucose in Tissue Prisms from Aging Rat Brain
Author(s) -
Sims N. R.,
Marek K. L.,
Bowen D. M.,
Davison A. N.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08654.x
Subject(s) - neocortex , acetylcholine , hippocampus , striatum , atropine , cholinergic , choline , endocrinology , medicine , chemistry , carbon dioxide , biology , neuroscience , organic chemistry , dopamine
Production of [ 14 C]acetylcholine and 14 CO 2 was examined by using tissue prisms from neocortex, hippocampus, and striatum from rats aged approximately 5 months, 13 months, and 27 months. [ 14 C]Acetylcholine synthesis in the striatum showed highly significant decreases with age for measurements in the presence of both 5 m m ‐ and 31 m m ‐K + , contrasting with the lack of significant change in 14 CO 2 production in this region. The neocortex and hippocampus showed only small changes, especially when comparison was made between 13‐month and senescent animals. Measurements of the release of [ 14 C]acetylcholine and influence of atropine on this release confirmed the relative stability with age of the cholinergic system in the neocortex.