Premium
Fluorometrical Analysis of Guanidino Compounds in Human Cerebrospinal Fluid
Author(s) -
Mori Akitane,
Watanabe Yoko,
Fujimoto Noboru
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08649.x
Subject(s) - chemistry , arginine , cerebrospinal fluid , chromatography , creatinine , high performance liquid chromatography , biochemistry , medicine , endocrinology , amino acid
Guanidino compounds in CSF of 57 human subjects were determined fluorometrically after reaction with phenanthrenequinone in alkali solution, using HPLC. Creatinine (65.2 ± 13.4 nmol/ml), arginine (24.7 ± 6.4 nmol/ml), and homoarginine (0.7 ± 0.3 nmol/ml) were found in all subjects. Trace amounts of guanidinosuccinic acid and guanidinoacetic acid were detected in some of the subjects. Brain guanidino compounds, taurocyamine, N ‐acetylarginine, and methylguanidine were not detected in CSF.