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Solid‐Phase Immunoassays for Quantitation of Antibody to Bovine White Matter Proteolipid Apoprotein
Author(s) -
Macklin Wendy B.,
Lees Marjorie B.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08635.x
Subject(s) - antibody , radioimmunoassay , chemistry , chromatography , enzyme , peroxidase , horseradish peroxidase , myelin proteolipid protein , microbiology and biotechnology , biochemistry , biology , myelin , immunology , central nervous system , endocrinology , myelin basic protein
Two solid‐phase immunoassays have been developed for quantitation of antibodies to bovine white matter proteolipid apoprotein. Conditions were established for optimal specific antibody binding. Water‐soluble proteolipid apoprotein was bound to microtiter plates and plates were incubated with test serum. Goat anti‐rabbit IgG conjugated with horeseradish peroxidase was used as the second antibody for an enzyme‐linked immunospecific assay and 125 I‐labeled protein A for a radioimmunoassay. Both procedures have been used to follow the time course of anti‐proteolipid antibody production in rabbits and to compare different immunization protocols.