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Effects of Divalent Cations on the Glycolipids from Cultured Mouse Neuroblastoma Cells
Author(s) -
Bremer Eric G.,
Sapirstein Victor S.,
Savage Tom,
McCluer Robert H.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08633.x
Subject(s) - lactosylceramide , egta , divalent , glycosphingolipid , chemistry , glycolipid , chelation , biochemistry , intracellular , ganglioside , calcium , organic chemistry
The influence of divalent cations on glycosphingolipid metabolism was examined in the NB41A mouse neuroblastoma clonal cell line. HPLC methods were utilized to quantitate the effects on neutral glycolipids and monosialogangliosides. NB41A cells were shown to contain G M3 , G M2 , G M1 , G D3 , and G D1a by HPLC and TLC. The neutral glycosphingolipids consisted of glucosylceramide (GlcCer), lactosylceramide (LacCer), GaINAc(β1→4) Gal(β1→4)Glc(β1→1)Cer (GgOse 3 Cer), and GaINAc(β1→3)Gal(α1→4) Gal‐(β1→4)Glc(β1→1)Cer (GbOse 3 Cer) according to their HPLC behavior. Cells grown in the presence of 1.85 m m ‐EGTA showed a two‐ to threefold increase in G M3 whereas other glycosphingolipids were only slightly affected. When cells were grown in the presence of 1.45 m m ‐EGTA plus 0.4 m m ‐EDTA a similar increase in G M3 was observed but this change was now accompanied by decreases in G M2 , G M1 GgOse 3 Cer, and GbOse 4 Cer. The EGTA‐EDTA effects were reversed when growth was in the presence of Ca 2+ sufficient to bind all chelator. Mn 2+ replacement reversed the chelator effects differentially; G M2 and G M1 levels were the most sensitive to increases in Mn 2+ concentration; GgOse 3 Cer and GbOse 4 Cer were also sensitive, whereas G M3 was the least affected. These results suggest calcium serves an important regulatory role on G M3 levels and that manganese concentration may regulate the levels of galactosamine‐containing glycolipids in mouse NB41A neuroblastoma cells.

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