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[ 3 H]Imipramine Is Accumulated but Not Released from Slices of the Rabbit Caudate and Hypothalamus
Author(s) -
Langer S. Z.,
Galzin A. M.,
Kamal L. A.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08630.x
Subject(s) - imipramine , potassium , dopamine , endocrinology , stimulation , chemistry , medicine , hypothalamus , depolarization , calcium , tyramine , lateral hypothalamus , biophysics , biology , alternative medicine , organic chemistry , pathology
Slices of rabbit caudate and hypothalamus take up and accumulate [ 3 H]imipramine. In superfused slices of both structures electrical stimulation or exposure to tyramine failed to release recently taken up [ 3 H]imipramine. De‐polarization by exposure to 30–60 mm‐potassium caused only a small release of [ 3 H]imipramine that was not concentration‐dependent. The release of [ 3 H]imipramine by high potassium was independent of the presence of calcium ions in the superfusion medium. These results contrasted with those obtained for the release of [ 3 H]dopamine from the caudate and [ 3 H]noradrenaline from the hypothalamus, where tyramine, electrical stimulation, and high potassium caused a significant release of the labeled neurotransmitters. The release of [ 3 H]dopamine from the caudate and [ 3 H]noradrenaline from the hypothalamus elicited by electrical stimulation or high potassium was entirely calcium‐dependent. It is concluded that [ 3 H]imipramine is taken up into the two brain regions and is accumulated in a nonvesicular site from which it is not released by calcium‐dependent depolarizing stimuli.