Premium
In Vivo Incorporation of [ 32 P]Orthophosphate into Myelin Basic Protein of Developing Rabbit Brain: Its Location in Components 3 and 5 and in a New Protein Tentatively Identified as Basic Protein Component 7
Author(s) -
Agrawal Harish C.,
Martenson Russell E.,
Agrawal Daya
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08016.x
Subject(s) - myelin basic protein , myelin , electrophoresis , phosphate , chemistry , polyacrylamide gel electrophoresis , covalent bond , in vivo , biochemistry , major basic protein , polyacrylamide , chromatography , biology , enzyme , central nervous system , immunology , endocrinology , organic chemistry , eosinophil , asthma , microbiology and biotechnology , polymer chemistry
Myelin was isolated from the brains of 16‐day‐old rabbits that had received intracerebral injections of [ 32 P]orthophosphate 24 h earlier. The basic protein was extracted and examined by polyacrylamide gel electrophoresis both at low and at high pH. At low pH a single band corresponding to the basic protein contained all of the covalently bound protein radioactivity. At high pH, three bands of radioactivity corresponding to basic protein components 3, 5, and 7 were observed. Estimation of the specific radioactivities of the components in conjunction with their high pH electrophoretic mobilities indicated that components 3, 5, and 7 contained one, two, and three phosphate groups per molecule of protein, respectively.