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An Improved Filtration Procedure for Measuring Opiate Receptors in Small Regions of Rat Brain
Author(s) -
Bardo M. T.,
Bhatnagar R. K.,
Gebhart G. F.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb08015.x
Subject(s) - opiate , receptor , (+) naloxone , opiate receptors , filtration (mathematics) , in vitro , ligand (biochemistry) , competitive binding , binding site , chemistry , radioligand assay , medicine , endocrinology , biology , biophysics , biochemistry , antagonist , statistics , mathematics
A modified filtration method for in vitro receptor binding was used to determine specific binding of [ 3 H]naloxone to small regions of adult rat brain. Reliable determinations of ligand binding were quantified with about 50 μ g of protein per assay tube. Large differences in [ 3 H]naloxone binding were obtained between various brain nuclei, and these differences were consistent with prior determinations of opiate receptor densities in various rat brain nuclei using autoradiographic techniques.