Premium
The Distribution of Radioligand Binding Activity and 5′‐Nucleotidase Activity in Bovine Caudate Nucleus Subcellular Fractions
Author(s) -
Withy Raymond M.,
Mayer R. John,
Strange Philip G.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb07912.x
Subject(s) - radioligand , chemistry , caudate nucleus , microsome , 5' nucleotidase , cell fractionation , spiperone , biochemistry , binding site , biology , dopamine receptor , endocrinology , enzyme , receptor
The distribution of 5′‐nucleotidase activity, dopaminergic [ 3 H]spiperone binding sites, and [ 3 H]quinuclidinyl benzilate (QNB) binding sites in different subcellular fractions of bovine caudate nucleus has been studied. Each activity was enriched in a microsomal (P 3 ) preparation from that tissue. The microsomal preparation was further fractionated by different techniques. First, the P 3 fraction, or a sonicated P 3 fraction, was fractionated on a discontinuous sucrose density gradient. Second, the P 3 fraction, or a digitonin pretreated P 3 fraction, was fractionated on a continuous sucrose density gradient. The results obtained demonstrate that 5′‐nucleotidase activity does not cofractionate with radioligand binding activity, although no difference between the distributions of [ 3 H]spiperone binding and [ 3 H]QNB binding were seen. It is concluded that the two radioligand binding activities are located on nonglial membranes.