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Cysteine Sulfinic Acid in the Central Nervous System: Uptake and Release of Cysteine Sulfinic Acid by a Rat Brain Preparation
Author(s) -
Iwata Heitaroh,
Yamagami Satoru,
Mizuo Hiroyuki,
Baba Akemichi
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb07900.x
Subject(s) - cysteic acid , chemistry , cysteine , sulfinic acid , veratridine , biochemistry , sodium , cystine , organic chemistry , sodium channel , enzyme
Uptake and release of cysteine sulfinic acid by synaptosomal fractions (P 2 ) and slices of rat cerebral cortex were investigated. The P 2 fraction had a Na + ‐dependent high‐affinity uptake system for cysteine sulfinic acid (K m , 12μM), which was restricted to the synaptosomes. High‐affinity uptake of cysteine sulfinic acid was competitively inhibited by glutamate, aspartate, and cysteic acid. None of the various centrally acting drugs tested specifically inhibited this transport system. Release of [ 14 C]cysteine sulfinic acid from preloaded cortical slices or P 2 fractions was examined by a superfusion method, which avoided reuptake of released [ 14 C]cysteine sulfinic acid. High K + (56 m M ) and veratridine (10μM) stimulated the release of cysteine sulfinic acid from slices and the P 2 fraction in a partly Ca 2+ ‐dependent manner. Diazepam at concentrations of 10 and 100 μM markedly inhibited the stimulated release, but not the spontaneous release, by cortical slices. On the contrary, it had no effect on the stimulated release of cysteine sulfinic acid from the P 2 fraction.