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The Kinetics of Acetylcholine Turnover in a Resting Cholinergic Nerve Terminal and the Magnitude of the Cytoplasmic Compartment
Author(s) -
Weiler M.,
Roed I. S.,
Whittaker V. P.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb07889.x
Subject(s) - acetylcholine , torpedo , compartment (ship) , choline , cholinergic , chemistry , cytosol , biophysics , kinetics , biochemistry , endocrinology , biology , acetylcholine receptor , oceanography , receptor , physics , quantum mechanics , geology , enzyme
The magnitude of the cytosolic pool of acetylcholine in the cholinergic electromotor nerve terminals of Torpedo marmorata has been calculated to be 22 ± 3% of the total by comparing the isotopic ratio of acetylcholine with that of choline when slices of electric organ were incubated with 10 μM deuterated choline. The calculation is based on a two‐compartment model that assumes the presence, in unstimulated tissue, of a vesicular pool of acetylcholine that does not exchange, under resting conditions, with a second cytosolic pool; the latter, by contrast, is subject to ‘futile recycling’ and comes into isotopic equilibrium with the tissue choline pool.