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In Vivo Biosynthesis of 35 S‐Substance P from [ 35 S]Methionine in the Rat Striatum and Its Transport to the Substantia Nigra
Author(s) -
Torrens Y.,
Michelot R.,
Beaujouan J. C.,
Glowinski J.,
Bockaert J.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb06655.x
Subject(s) - substantia nigra , striatum , methionine , in vivo , biosynthesis , chemistry , biochemistry , endocrinology , biology , dopamine , enzyme , amino acid , genetics , dopaminergic
Rats with a push‐pull cannula implanted in the right striatum were used to study the biosynthesis of 35 S‐substance P (SP) from [ 35 S]methionine and its transport to the ipsilateral substantia nigra. [ 35 S]Methionine was delivered for 2, 3 or 5 h to the push‐pull cannula. 35 S‐SP in striatal and nigral tissues was estimated after immunoadsorption and HPLC. Higher levels of 35 ‐SP in striatal homogenates were found after a 5‐h labelling period. 35 S‐SP biosynthesis was inhibited when cycloheximide was superfused together with [ 35 S]methionine. The identity of 35 S‐SP was further checked by its conversion into 35 S‐SP sulphoxide. After a 5‐h labelling period, 35 S‐SP was also recovered in the substantia nigra. This was not the case after hemisection of striato‐nigral fibers. When rats were killed 15 or 24 h after the 5‐h labelling period, 35 S‐SP levels in the substantia nigra were higher than those found just after the 5‐h labelling period, while the reverse was observed in the striatum.