Premium
Calcium‐Dependent Proteases in Neuroblastoma Cells
Author(s) -
DeMartino George N.,
Croall Dorothy E.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb06645.x
Subject(s) - proteases , calcium , enzyme , chemistry , biochemistry , neuroblastoma , enzyme assay , biology , cell culture , genetics , organic chemistry
Extracts of neuroblastoma cells contained calcium‐dependent proteolytic activity. This activity was accounted for by two distinct proteases. These proteases were separated by ion‐exchange chromatography. Although each was totally dependent on calcium, the calcium requirements of the enzymes were different; one enzyme required approximately 40 μM Ca 2+ for half‐maximal activity and the other enzyme required approximately 150 μM Ca 2+ for half‐maximal activity.