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Identification of a Cholinergic‐Specific Antigen Chol‐1 as a Ganglioside
Author(s) -
Richardson Peter J.,
Walker John H.,
Jones R. Theresa,
Whittaker Victor P.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb06640.x
Subject(s) - antiserum , antigen , ganglioside , antibody , cholinergic , microbiology and biotechnology , immunohistochemistry , biology , torpedo , neuraminidase , biochemistry , chemistry , immunology , acetylcholine receptor , endocrinology , receptor , enzyme
An antiserum specific for cholinergic terminals was used to identify an antigen conserved between Elasmobranchs and mammals. Immunohistochemistry and a cytotoxicity test were used to assay the binding of antibody to mammalian terminals. Torpedo electric organ gangliosides totally abolished antibody binding. The highest inhibitory activity was associated with a single polysialoganglioside band on TLC plates. Neuraminidase altered the migration of the inhibitory activity on TLC plates. Antibody binding was inhibited by ganglioside fractions derived from chicken and mammalian brains. A summary of those tissues in which the antigen has been detected is presented. The possible function of the antigen is discussed.