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Lectin‐Reactive Components in White Matter Membranes from Normal and Multiple Sclerosis Brains
Author(s) -
Hukkanen Veijo
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb06630.x
Subject(s) - wheat germ agglutinin , concanavalin a , lectin , ricinus , agglutinin , gel electrophoresis , molecular mass , soybean agglutinin , biochemistry , white matter , membrane , polyacrylamide gel electrophoresis , chemistry , microbiology and biotechnology , biology , enzyme , medicine , radiology , magnetic resonance imaging , in vitro
Abstract: Polypeptides derived from human white matter membranes reacted with the radioiodinated lectins concanavalin A, Lens culinaris phytohemagglutinin, Ricinus communis agglutinin and wheat germ agglutinin after electrophoresis in polyacrylamide pore gradient gels. The molecular weights of these lectin‐reactive bands were estimated by comparison with radioiodinated protein standards by using the linear relationship between log of the molecular weight and log of the gel concentration reached by the protein after electrophoresis in a polyacrylamide gradient gel. The molecular weight estimates for components reactive with concanavalin A were 176,800, 141,200, 72,800, 52,800, 44,700, 40,000, 24,800 and 23,900. The molecular weights of the bands reactive with both wheat germ agglutinin and Lens culinaris phytohemagglutinin were 138,000, 113,500, 92,100, 52,800, 44,700, 24,800 and 23,900. Wheat germ agglutinin was bound also to a band with a molecular weight of 72,800. Ricinus communis agglutinin bound to bands with estimated molecular weights of 138,000, 72,800, 52,800, 44,700, 24,800 and 23,900. The electrophoretic pattern of lectin‐reactive polypeptides derived from normal‐appearing white matter of multiple sclerosis brains was not qualitatively different from the lectin‐binding pattern of control brain membrane polypeptides.