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Tyrosine Hydroxylase Activity in Rat Brain Synaptosomes: Direct Measurement Using High Performance Liquid Chromatography
Author(s) -
Messripour Manoucher,
Clark John B.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb05360.x
Subject(s) - tyrosine hydroxylase , chemistry , tyrosine , chromatography , synaptosome , dopamine , striatum , specific activity , enzyme , tyrosine 3 monooxygenase , substrate (aquarium) , forebrain , enzyme assay , biochemistry , central nervous system , endocrinology , biology , in vitro , ecology
By use of high performance liquid chromatography with electrochemical detection to measure dopamine production, tyrosine hydroxylase (EC 1.14.16.2) activity has been measured in rat brain synaptosomes from striatum and forebrain. Normal specific activities three‐ to fivefold higher than previously reported in the literature for radiochemical methods of assay were found. It is suggested that synaptosomes contain a significant amount of endogenous substrate for tyrosine hydroxylase, which causes dilution of the added labelled tyrosine and hence underestimation of the activity of this enzyme when radiochemical methods are used.