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Adenosine Measurement by a Rapid HPLC‐Fluorometric Method: Induced Changes of Adenosine Content in Regions of Rat Brain
Author(s) -
Wojcik W. J.,
Neff N. H.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb04736.x
Subject(s) - adenosine , hippocampus , high performance liquid chromatography , striatum , thalamus , chemistry , cerebral cortex , midbrain , cortex (anatomy) , fluorescence , chromatography , biology , endocrinology , neuroscience , biochemistry , central nervous system , dopamine , physics , quantum mechanics
We describe a rapid, sensitive method to determine brain adenosine content by HPLC. Adenosine is first reacted with chloroacetaldehyde to form fluorescent 1, N 6 ‐ethenoadenosine. The derivative is then separated from interfering compounds by HPLC on a C18 reverse‐phase column and quantitated by fluorometry. We found that adenosine was rather uniformly distributed in nine brain regions of animals killed by microwave radiation. In contrast, there was an increase of adenosine in hippocampus, frontal cortex, and especially striatum of animals killed by decapitation. Moreover, adenosine content increased approximately 10‐fold in the thalamus, mesencephalon, and pons‐medulla if the animals were exposed to CO 2 for 1 min before they were killed by microwave radiation. Our method should be a useful aid for providing new information about the metabolic and proposed transmitter roles of brain adenosine.