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Application to Mammalian Tissues of the Chemiluminescent Method for Detecting Acetylcholine
Author(s) -
Israël Maurice,
Lesbats Bernard
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb04727.x
Subject(s) - acetylcholine , chemiluminescence , neuroscience , chemistry , computational biology , biology , chromatography , pharmacology
It is now possible to extend to mammalian tissues the chemilumi‐nescent acetylcholine assay. Mammalian tissue extracts must be treated with oxidants (which is not necessary for electric organ extracts). The assay can then be performed as previously described (acetylcholinesterase hydrolyses acetylcholine; choline oxidase converts choline to betaine and H 2 O 2 , which gives off light in the presence of luminol and peroxidase). It is also shown that release experiments can be performed on mammalian tissue slices (mouse caudate nucleus) after the slice is washed in oxygenated saline solutions.