Premium
Pharmacological and Electrophysiological Characterization of Lithium Ion Flux Through the Action Potential Sodium Channel in Neuroblastoma × Glioma Hybrid Cells
Author(s) -
Reiser G.,
Scholz F.,
Hamprecht B.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb04723.x
Subject(s) - veratridine , tetrodotoxin , sodium channel , biophysics , electrophysiology , neuroblastoma , chemistry , voltage gated ion channel , sodium , glioma , membrane potential , stimulation , scorpion toxin , ion channel , cell culture , biology , biochemistry , neuroscience , receptor , genetics , organic chemistry , cancer research
Interaction of Li + with the voltage‐dependent Na + channel has been analyzed in neuroblastoma × glioma hybrid cells. The cells were able to generate action potentials in media containing Li + instead of Na + . The uptake of Li + into the hybrid cells was investigated for the pharmacological analysis of Li + permeation through voltage‐dependent Na + channels. Veratridine and acionitine increased the uptake of Li + to the same degree (EC 50 30 μ M ). This increase was blocked by tetrodotoxin (IC 50 20 n M ). Veratridine and aconitine did not act synergistically; however, the veratridine‐stimulated influx was further enhanced by the toxin of the scorpion Leiurus quinquestriatus (EC 50 0.06 μg/ml). This stimulation was also blocked by tetrodotoxin. Thus, the voltage‐dependent Na + channel of the hybrid cells accepts both Li + and Na + in a similar manner.