Premium
Effects of Pentobarbitone on 45 Ca 2+ Transport by Rat Brain Mitochondria
Author(s) -
Willow Max,
Bygrave Fyfe L.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb03981.x
Subject(s) - ruthenium red , egta , mitochondrion , barbiturate , chemistry , efflux , biophysics , stimulation , calcium , dibucaine , isolated brain , membrane potential , biochemistry , pharmacology , biology , endocrinology , organic chemistry
The effects of pentobarbitone on the transport of 45 Ca 2+ by rat brain mitochondria were studied, using the Ruthenium Red‐EGTA quench technique. In the presence of succinate and inorganic phosphate, mitochondria rapidly accumulate 45 Ca 2+ . Pentobarbitone (0.1–1.0 m M ) stimulates the initial rate of Ca 2+ transport. In contrast, pentobarbitone (1 m M ) did not affect the NaCl (50 m M )‐induced efflux of 45 Ca 2+ from mitochondria. Dibucaine (60 μM ), a clinically used local anaesthetic, inhibits both 45 Ca 2+ uptake and efflux. The results suggest that barbiturate stimulation of mitochondrial Ca 2+ uptake may, in combination with effects on other Ca 2+ sequestering processes, contribute to the inhibition of transmitter release observed at a number of synapses.