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5‐Hydroxytryptamine Stimulates [ 3 H]Dopamine Release from the Fish Retina
Author(s) -
Kato S.,
Teranishi T.,
Kuo C. H.,
Negishi K.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb03971.x
Subject(s) - methysergide , dopamine , serotonin , spiperone , endocrinology , medicine , chemistry , tryptamine , dopaminergic , neurotransmitter , carp , 5 ht receptor , biophysics , biology , receptor , fish <actinopterygii> , biochemistry , dopamine receptor , fishery
Serotonin (5‐hydroxytryptamine, 5‐HT; 0.5 μM and above) stimulated the release of [ 3 H]dopamine ([ 3 H]DA) from particulate fractions of the carp ( Cyprinus carpio ) retina. The 5‐HT effect was dose‐ and Ca 2+ ‐dependent, and was structurally specific. A similar response was not elicited by the other indoles (5,6‐dihydroxytryptamine, 5,7‐dihydroxytryptamine, 5‐hydroxytrypto‐phan, or 5‐hydroxyindoleacetic acid) examined. An increase in [ 3 H]DA release was elicited by addition of 5‐HT agonists (5‐methoxytryptamine, 5‐methoxy‐ N,N‐ dimethyltryptamine, and tryptamine), but not antagonized by three 5‐HT antagonists (metergolin, methysergide, and spiperone). Either DA alone or noradrenaline (0.5 m M ) produced a large increase in [ 3 H]DA release from the particulate fractions, but this action was Ca 2+ ‐independent. Further, no significant release of [ 3 H]γ‐aminobutyric acid could be evoked by 5‐HT (0.5 mM) under similar experimental conditions. Taken together, the present data suggest that 5‐HT stimulates [ 3 H]DA release from the fish retina through a specific receptor‐mediated mechanism on dopaminergic terminals, but not through an exchange or nonspecific phenomenon.