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Increased Nervous System‐Specific Enolases in Rat Plasma and Cerebrospinal Fluid in Bilirubin Encephalopathy Detected by an Enzyme Immunoassay
Author(s) -
Semba Reiji,
Kato Kanefusa
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb03956.x
Subject(s) - cerebrospinal fluid , enolase , central nervous system , kernicterus , alpha (finance) , cisterna magna , immunoassay , gamma glutamyltransferase , encephalopathy , isozyme , nervous system , pathology , endocrinology , medicine , enzyme , chemistry , biology , bilirubin , biochemistry , immunology , antibody , immunohistochemistry , neuroscience , construct validity , nursing , patient satisfaction
Three forms of enolase isozymes (αα, αγ, and γγ), including nervous system‐specific forms, were measured in the cerebrospinal fluid and the blood plasma of jaundiced or nonjaundiced infant rats by means of enzyme immunoassay systems capable of detecting each form of enolase at the 1 amol (10 −18 mol) level. Average enolase levels in cerebrospinal fluid in normal rat were 2.0, 0.2 and 0.1 pmol/ml for αα, αγ, and γγ forms, respectively. Levels of αγ and γγ forms (nervous system‐specific enolases; NSE) in jaundiced rats, which suffer Purkinje cell degeneration due to the inborn hyperbilirubinemia, were three to four times as high as the normal values. When kernicterus was induced in jaundiced rats by an injection of bucolome, the NSE level in cerebrospinal fluid was elevated up to more than 30‐fold the control, together with a significantly higher level of αγ form in blood plasma. These results suggest that assays of NSE in the cerebrospinal fluid or the blood plasma are helpful in detecting neuronal damage in the central nervous system.