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HPLC Analysis of Neurophysin Proteins from Neurosecretory Granules
Author(s) -
Schwandt P.,
Richter W. O.,
ReuschelJanetschek E.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb03955.x
Subject(s) - neurophysins , isoelectric focusing , posterior pituitary , biochemistry , chromatography , chemistry , polyacrylamide gel electrophoresis , amino acid , isoelectric point , pituitary gland , biology , hormone , enzyme
Neurosecretory granules were obtained from neurolobes of porcine pituitary glands. From the granules, highly purified neurophysins were prepared by HPLC. According to polyacrylamide gel electrophoresis, isoelectric focusing, N‐ and C‐terminal amino acid residue determination, and amino acid composition, the neurophysins I 1 I 2 , and II were identical to the neurophysins obtained from whole posterior lobes. Since degradation could not have occurred, we conclude that neurophysin I 1 and I 2 originated in the neurosecretory granules.