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Fatty Acid Activation and Temperature Perturbation of Rat Brain Microsomal Phospholipase D
Author(s) -
Chalifour Robert,
Kanfer Julian N.
Publication year - 1982
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1982.tb03946.x
Subject(s) - phospholipase , chemistry , phospholipase a , arrhenius plot , phospholipase a1 , hydrolysis , activator (genetics) , phospholipase a2 , incubation , phospholipase c , biochemistry , phospholipase d , enzyme , microsome , activation energy , organic chemistry , gene
The hydrolytic and transphosphatidylation activities of rat brain microsomal phospholipase D were highly latent in the absence of an appropriate activator. The most suitable surfactants for this activation were oleate and palmitoleate. Besides the bile acids and unsaturated fatty acids, other naturally occurring surfactants, such as lysophospholipids, acidic phospholipids, acyl‐CoA's, and gangliosides, were inactive. Taurodeoxycholate, at optimal concentration, produced a profound inhibition of oleate activation. Phospholipase D activity was detectable in all rat tissues investigated. The optimal incubation temperature for phospholipase D was 30°C, with a break point at 16.1°C in an Arrhenius plot.