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Studies on the Interaction of Ca 2+ Ions with Some Fractions of the Neurospecific S‐100 Protein
Author(s) -
Starostina M. V.,
Malup T. K.,
Sviridov S. M.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb10814.x
Subject(s) - chemistry , calcium , calcium binding protein , fluorescence , ion , biophysics , fluorescence spectroscopy , crystallography , biology , organic chemistry , physics , quantum mechanics
Fractions of neurospecific S‐100 protein were purified from bovine brain and their physicochemical properties were studied. Conformational changes caused by the binding of calcium to S‐100 protein fractions were detected by means of differential and fluorescence spectroscopy. Fractions demonstrating opposite shifts of their spectra also differ in the distribution in double‐phase system. The number of calcium‐binding centers and their association constants were determined by means of equilibrium dialysis and gel filtration. The nature of the differences in the interaction of various S‐100 protein fractions with calcium is discussed.