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Subcellular and Perisynaptic Distribution of Rat Brain Aldehyde Dehydrogenase Activity
Author(s) -
Koivula Timo,
Turner Anthony J.,
Huttunen Matti,
Koivusalo Martti
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb10812.x
Subject(s) - aldehyde dehydrogenase , aldh2 , biochemistry , catecholaminergic , branched chain alpha keto acid dehydrogenase complex , adh1b , chemistry , enzyme , dopamine , biology , dehydrogenase , endocrinology
The nuclear mitochondrial and synaptosomal fractions of rat brain were each found to contain some 25–30% of the total aldehyde dehydrogenase activity. The cytoplasmic fraction had a very low total aldehyde dehydrogenase activity. There were differences in the distribution of the activity when different aldehydes were used as substrates, suggesting the presence of isoenzymes in the various subcellular compartments. When rats were treated intra‐cisternally with 6‐hydroxydopamine there was no change in brain aldehyde dehydrogenase activity, although the noradrenaline content and the activities of tyrosine hydroxylase and dopamine‐β‐hydroxylase were markedly decreased. Treatment with 6‐hydroxydopamine also had no significant effect on the aldehyde dehydrogenase activity in retinal homogenates. The results suggest that the aldehyde dehydrogenase activity in rat brain is predominantly outside the catecholaminergic nerve terminals.