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Alternative Pathways of Glucose Utilization in Brain; Changes in the Pattern of Glucose Utilization in Brain Resulting from Treatment of Rats with 6‐Aminonicotinamide
Author(s) -
Hothersall John S.,
Zubairu Salmata,
McLean Patricia,
Greenbaum A. Leslie
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb06318.x
Subject(s) - pentose phosphate pathway , citric acid cycle , biochemistry , glycolysis , metabolic pathway , metabolism , tricarboxylic acid , glutamate receptor , metabolite , gluconeogenesis , chemistry , carbohydrate metabolism , biology , pentose , medicine , receptor , fermentation
The effect of 6‐aminonicotinamide (6AN) treatment on the activities of alternative pathways of glucose metabolism in 20‐day‐old rat brain was evaluated by measurements of yields of 14 CO 2 from glucose labeled with 14 C on carbons 1, 2, 3 + 4, or 6 and uniformly labeled glucose, and from the incorporation of 14 C from specifically labeled glucose into lipids by brain slices from cerebral hemispheres and cerebellum. At the highest dose of 6AN used (35 mg/kg body weight) there was a significant decrease in the 14 CO 2 yields via the pentose phosphate pathway, the glycolytic route, tricarboxylic acid (TCA) cycle, and via the glutamate‐ γ ‐aminobutyric acid pathway. Giving a graded series of doses (20–35 mg 6AN/kg body weight) revealed a hierarchy of responses in which the pentose phosphate pathway, lactate, glyceride‐glycerol, and fatty acid formation were most sensitive, followed, in sequence, by the pyruvate dehydrogenase reaction, the glutamate‐ γ ‐aminobutyrate route and, finally, the TCA cycle. The nature of the blocks in the various pathways was examined by the use of metabolite profiles.