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Phosphate Ion Transport in Rabbit Brain Synaptosomes
Author(s) -
Salamin A.,
Deshusses J.,
Straub R. W.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb06310.x
Subject(s) - nigericin , phosphate , ouabain , chemistry , sodium , potassium , biophysics , vesicle , monensin , ion transporter , membrane , membrane transport , valinomycin , biochemistry , biology , organic chemistry
Synaptosomes (vesicles of nerve endings) isolated from rabbit brain were studied as a model system for the uptake of inorganic phosphate. The phosphate uptake showed a sodium‐dependent, saturable component with a K t of 0.29 m m , The sodium‐dependent component was larger at pH 6 than at pH 7.4 or 8. Application of potassium salts, ouabain, monensin, nigericin or FCCP decreased the uptake. The results indicate that the sodium‐sensitive phosphate influx is dependent on the Na + gradient and on the membrane potential, which might act, preferentially, on the transport of the monovalent phosphate ion.