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Protection of Neocortical Tissue Prisms from Freeze‐Thaw Injury by Dimethyl Sulphoxide
Author(s) -
Haan E. A.,
Bowen D. M.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb05315.x
Subject(s) - chemistry , neuroscience , biophysics , biology
Neocortical tissue prisms prepared from rat and human brain were frozen to ‐196°C by a two‐step freezing procedure and 10% dimethyl sulphoxide as cryoprotectant. Frozen and thawed rat neocortical prisms incorporated glucose into acetylcholine and carbon dioxide at 89% and 86% of control values, respectively, and noradrenaline uptake into frozen and thawed rat prisms was 94% of the control value. Frozen and thawed prisms from three human neocortical specimens showed a similar degree of protection from freeze‐thaw injury.