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2‐Deoxyglucose Incorporation in the Cerebellum of Weaver and Nervous Mutant Mice
Author(s) -
Mikoshiba Katsuhiko,
Kohsaka Shinichi,
Takamatsu Ken,
Tsukada Yasuzo
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb05307.x
Subject(s) - cerebellum , granule cell , granular layer , purkinje cell , deoxyglucose , biology , neuroscience , nervous system , cerebellar cortex , central nervous system , white matter , biochemistry , dentate gyrus , medicine , radiology , magnetic resonance imaging
The 2‐deoxyglucose autoradiographic method has been used to study activity in cerebellum of the weaver and nervous mutant mice. Patterns of 2‐deoxyglucose incorporation into the cerebral hemispheres from weaver and nervous strains did not differ significantly from those of the controls. In the normal cerebellum, 2‐deoxyglucose incorporation was maximal in the granular layer, where mossy fibers form synapses with the dendrites of granule cells. In the cerebellum of nervous mice, which lacks Purkinje cells, the incorporation of the 2‐deoxyglucose was maximal in the granular layer, but the incorporation into the molecular layer appeared less than in the control. The incorporation into the cerebellum from weaver, which lacks granule cells, was much higher than that of the control, the maximal incorporation being found in the Purkinje cell layer and in cell masses located in the white matter. These data suggest that the heterologous synapses that mossy fibers or climbing fibers form with the cells in the Purkinje cell layer and the cells in the white matter in the weaver cerebellum are functional.