Premium
Biphasic Modulation by ACTH‐Like Peptides of Protein Synthesis in a Cell‐Free System from Rat Brain
Author(s) -
Schotman Peter,
Allaart Jeanne
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb04690.x
Subject(s) - aurintricarboxylic acid , peptide , chemistry , stimulation , protein biosynthesis , cell , biochemistry , endocrinology , medicine , biophysics , biology , apoptosis , programmed cell death
— Brain protein synthesis in a cell‐free system was stimulated by 10 8 m ‐ACTH 1‐24 . This stimulatory effect was completely inhibited by aurintricar‐boxylic acid (ATA), an inhibitor of reinitiation of new peptide chains. The N‐terminal peptide sequence 4‐ 10 exerted a biphasic modulation of cell‐free protein synthesis, i.e., a stimulation at low concentrations (10 −8 and 10 −10 m ) and an inhibition at a high concentration (10 −4 m ). The d ‐isomer, ACTH 4‐10 ‐7‐ d ‐phe, also showed a biphasic modulation that, however, was in a direction opposite to that shown by ACTH 4‐10 ‐7‐ l ‐pheat 10 −8 m and 10 −4 m .