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Cadaverine in the Rat Brain: Regional Distribution and Acylation of [ 14 C]Cadaverine In Vivo and Uptake In Vitro
Author(s) -
Salzman Steven K.,
StepitaKlauco Matej
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb04682.x
Subject(s) - cadaverine , cerebrum , chemistry , putrescine , in vivo , striatum , cerebellum , acylation , pons , biochemistry , medicine , endocrinology , central nervous system , dopamine , biology , anatomy , enzyme , microbiology and biotechnology , catalysis
— The regional distribution and acylation of intraventricularly injected [ 14 C]cadaverine was studied in the rat brain over a 48‐h period. The concentrations of labeled cadaverine and its acyl derivatives, N ‐monoacetylcadaverine and N ‐monopropionylcadaverine, were determined in the telencephalon, striatum, hypothalamus, midbrain, cerebellum, and medulla‐pons by TLC of their 5‐dimethylamino‐1‐naphthalenesulfonyl derivatives, followed by liquid scintillation spectrometry. The apparent passage of radioactivity from the ventricular space into brain tissue was slow, with the concentrations reaching a peak at 24 h after injection. The percentage of radioactivity in the acyl forms of cadaverine, however, was maximal 4 h after injection, with the propionyl form predominating. The telencephalon, striatum, and hypothalamus contained the highest concentrations of radioactivity, in all three forms, at all elapsed times. A high‐affinity uptake mechanism for cadaverine was demonstrated in slices of these tissues. This process was completely inhibited by equimolar concentrations of unlabeled putrescine.