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Characterization of Immunoreactive Thyrotropin Releasing Hormone in Human Fetal Cerebellum
Author(s) -
Parker C. Richard
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb04677.x
Subject(s) - thyrotropin releasing hormone , cerebellum , fetus , endocrinology , medicine , hormone , biology , pregnancy , genetics
— High concentrations (411 ± 30 pg/mg protein; mean ± S.E., n = 12) of immunoreactive TRH (TRH i ) were detected in extracts of human fetal cerebellum (13‐26 weeks gestation). The TRH i in the cerebellar extracts, when subjected to gel filtration and high performance liquid chromatography (HPLC), co‐migrated with synthetic TRH. In each instance, no TRH i was detected which did not share identical chromatographic mobilities with synthetic TRH. Like synthetic TRH, the TRH i in extracts of fetal cerebellum and hypothalamus was insoluble in diethyl ether and was efficiently degraded when incubated at 37°C with adult rat serum. No significant degradation of TRH i occurred when the incubation was conducted at 0°C or when the extracts were incubated with rat serum that had been preheated at 60°C for 20 min. Neither synthetic TRH nor TRH i in extracts of fetal cerebellum or hypothalamus was degraded when incubated with human umbilical cord serum at 37°C or 0°C. The results of this study are supportive of the view that the TRH i in extracts of human fetal cerebellum is identical to TRH.