Premium
Presence of Conjugated 5‐Hydroxytryptamine in In Vivo Superfusates of Rat Spinal Cord
Author(s) -
Hammond Donna L.,
Yaksh Tony L.,
Tyce Gertrude M.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb04501.x
Subject(s) - chemistry , in vivo , clorgyline , probenecid , spinal cord , monoamine oxidase , serotonin , pharmacology , medicine , endocrinology , biochemistry , enzyme , biology , neuroscience , receptor , microbiology and biotechnology
The presence of an acid‐hydrolyzable conjugate of 5‐hydroxytryptamine, presumably 5‐hydroxytryptamine‐ O ‐sulfate, was demonstrated in in vivo superfusates of rat spinal cord by HPLC with electrochemical detection. In untreated rats, the concentration of the 5‐hydroxytryptamine conjugate measured during the basal efflux of 5‐hydroxytryptamine did not differ from that measured during the release of 5‐hydroxytryptamine evoked by dl ‐ ρ ‐chloroamphetamine. Pretreatment of the rats with clorgyline, an inhibitor of the enzyme monoamine oxidase (EC 1.4.3.4), or with probenecid did not alter the concentrations of conjugated 5‐hydroxytryptamine measured during the basal efflux of 5‐hydroxytryptamine, but did elevate the concentrations of conjugate measured during the evoked release of 5‐hydroxytryptamine.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom