Premium
Experimental Galactose Toxicity: Effects on Synaptosomal Phosphatidylinositol Metabolism
Author(s) -
Berry Gerard,
Yandrasitz John R.,
Segal Stanton
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb04475.x
Subject(s) - phosphatidylinositol , inositol , acetylcholine , toxicity , endocrinology , medicine , galactose , chemistry , weaning , metabolism , biochemistry , biology , signal transduction , receptor
Experimental galactose toxicity was induced by weaning rats onto an isocaloric 40% galactose diet. Synaptosomes were prepared from cerebra of rats at 2‐9 weeks post‐weaning and incubated with [ 33 P]P i and myo ‐[2‐ 3 H]inositol in the presence or absence of 0.2 mM‐acetylcholine. The acetylcholine‐stimulated [ 33 P]P i labeling of phosphatidylinositol and the changes in amounts of phosphatidylinositol were similar in the normal and galactose‐toxic rats; however, acetylcholine‐stimulated myo ‐[2‐ 3 H]inositol labeling of phosphatidylinositol was markedly decreased in the galactose‐toxic rats. The impairment of acetylcholine‐stimulated myo ‐[2‐ 3 H]inositol incorporation into phosphatidylinositol observed after 2 weeks on the diet did not vary after more prolonged exposure to galactose.