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Molecular Forms of Acetylcholinesterase in Pineal Cell and Sympathetic Neuronal Cultures
Author(s) -
Rowe Ver,
Fernandez Hugo,
Fernandez Hugo,
Duell Myron,
Parr James,
Battie Cynthia
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb04472.x
Subject(s) - acetylcholinesterase , aché , cell culture , biology , neuron , medicine , pineal gland , endocrinology , neuroscience , microbiology and biotechnology , enzyme , biochemistry , melatonin , genetics
The activities of the various molecular forms of acetylcholinesterase (AChE) were measured in monolayer cultures of neonatal rat pineal cells grown alone and in co‐culture with sympathetic neurons. AChE forms characterized by sedimentation coefficients of 4S, 6.5S, and 10S were found in the neuronal and pineal cultures, as well as in the co‐cultures. The 16S AChE form was found only in the neuronal cultures. Total AChE activity increased with culture age in the co‐cultures, but it decreased in pineal cells cultured alone. The low level of activity present in the neuronal cultures did not change markedly over the 27‐day culture period. These results, which show bidirectional neuron‐pineal cell effects, suggest that AChE molecular forms may be important markers to study the mechanisms underlying neuron‐target cell interaction in the developing sympathetic nervous system.