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Partial Inactivation of Na,K‐ATPase in Cortical Brain Slices Incubated in Normal Krebs‐Ringer Phosphate Medium at 1 and at 10 Atm Oxygen Pressures
Author(s) -
Kovachich G. B.,
Mishra O. P.
Publication year - 1981
Publication title -
journal of neurochemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.75
H-Index - 229
eISSN - 1471-4159
pISSN - 0022-3042
DOI - 10.1111/j.1471-4159.1981.tb02418.x
Subject(s) - incubation , oxygen , chemistry , atpase , phosphate , oxidative phosphorylation , biochemistry , enzyme , organic chemistry
Na, K‐ATPase (ATP phosphohydrolase EC 3.6.1.3) activity was determined in homogenates of cortical brain slices after incubation in normal Krebs‐Ringer phosphate medium at 1 atm oxygen pressure. After 10 min of incubation Na,K‐ATPase activity was reduced by approximately 50%. Longer incubation did not cause further change in activity. The presence of 0.1 mM‐MnCl 2 in the medium offered significant protection. while an excursion to 10 atm oxygen pressure caused further inactivation. Measurements of malonaldehyde levels suggest that the inhibition of Na,K‐ATPase is a result of lipid peroxidation. The evidence indicates that brain slices incubated under standard conditions suffer considerable oxidative damage.